Programmed nuclear death and its relation to apoptosis and autophagy during sexual reproduction in Tetrahymena thermophila
نویسندگان
چکیده
Department of Biology, York University, Toronto M3J 1P3, Canada Geisel School of Medicine at Dartmouth, Dartmouth College, Lebanon NH 03756, USA Graduate School of Natural Science and Technology, Kanazawa University, Kanazawa 9201192, Japan Department of Biodiversity Science, Division of Biological Resource Science, Graduate School of Agricultural Science, Tohoku University, Oosaki 989-6711, Japan
منابع مشابه
Role of ATG8 and autophagy in programmed nuclear degradation in Tetrahymena thermophila.
Autophagy is an evolutionarily conserved mechanism for the degradation of cellular components, but its role in enucleation during differentiation has not been established. Tetrahymena thermophila is a unicellular eukaryote with two functionally distinct nuclei, the somatic (macro-) and the germ line (micro-) nuclei. These nuclei are produced during sexual reproduction (conjugation), which invol...
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Programmed nuclear death (PND) in the ciliate protozoan Tetrahymena thermophila is a novel type of autophagy that occurs during conjugation, in which only the parental somatic macronucleus is destined to die and is then eliminated from the progeny cytoplasm. Other coexisting nuclei, however, such as new micro- and macronuclei are unaffected. PND starts with condensation in the nucleus followed ...
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Genetically programmed DNA rearrangements can regulate mRNA expression at an individual locus or, for some organisms, on a genome-wide scale. Ciliates rely on a remarkable process of whole-genome remodeling by DNA elimination to differentiate an expressed macronucleus (MAC) from a copy of the germline micronucleus (MIC) in each cycle of sexual reproduction. Here we describe results from the fir...
متن کاملSUMOylation is developmentally regulated and required for cell pairing during conjugation in Tetrahymena thermophila.
The covalent attachment of small ubiquitin-like modifier (SUMO) to target proteins regulates numerous nuclear events in eukaryotes, including transcription, mitosis and meiosis, and DNA repair. Despite extensive interest in nuclear pathways within the field of ciliate molecular biology, there have been no investigations of the SUMO pathway in Tetrahymena. The developmental program of sexual rep...
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Conjugation in Tetrahymena thermophila involves a developmental program consisting of three prezygotic nuclear divisions, pronuclear exchange and fusion, and postzygotic and exconjugant stages. The conjugation junction structure appears during the initiation of conjugation development, and disappears during the exconjugant stage. Many structural and functional proteins are involved in the estab...
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